Using reverse dot blotting technique, genotype of HLA DQA1 locus have been determined from 142 unrelated Korean individuals. Twenty genotypes were found from possible twenty one genotypes -the missing one was A2/A2 that had lowest expected
frequency.
All of known 6 alleles were found with each of its frequency being 15.1% for A1. 1, 16.6% for A1.2, 12.7% for A1.3, 11.6% for A2, 25.7% for A3 and 18.3% for A4. After X²-test(p>0.1), G-test(p>0.05), and by comparision of expected(0.82) and
observed
heterozygosity(0.81), the population was confirmed to be on the state of Hardy-Weinberg equilibrium. The gene diversity(0.82) of Korean population, which generally thought to be a group of single unity, actually was higher than that of most other
populations. The pattern of allelic distribution was different from that of other populations, especially allele A1.3 which displayed heterogeneity between other groups with significance(p<0.01),as it turned out to have anthropological
significance.
After all, this HLA DQA1 system, even though its small number of alleles, having high degree of heterozygosity, was proven to be effective in individual identifcation, and paternity testing in Korean population.
|